Different endocytic functions of AGEF-1 in C. elegans coelomocytes

► Reduced AGEF-1 levels in C. elegans results in Cup and lysosomal enlargement in CC. ► ARL-8 functions upstream of AGEF-1 in lysosomes but downstream in uptake. ► AGEF-1 may function in lysosomes independent of its ARF exchange activity. ► ARF-1 and ARF-3 are candidate proteins as AGEF-1 substrates for the Cup defect.