Plexin-B3 interacts with EB-family proteins through a conserved motif

► Microtubule end-binding EB proteins are novel interactors for plexins. ► EB proteins form complexes with plexin-A2, B1 and B3 but not with plexin-B2. ► Plexins that interact with EBs contain the conserved EB-binding motif SxIP. ► Plexin-B1 peptide containing the EB-binding motif modulates neurite growth.