The thermodynamic and binding properties of the transferrins as studied by isothermal titration calorimetry

► ITC is a valuable tool to characterize the thermodynamics of binding interactions. ► A large number of metal ions exhibit very high binding affinity to transferrin. ► Holo-hTf displays high affinity to TfR and favorable enthalpy and entropy changes. ► Both lobes of hTf appear to contribute to the thermodynamics of hTf-TfR interaction.