The interplay of the EIIANtr component of the nitrogen-related phosphotransferase system (PTSNtr) of Pseudomonas putida with pyruvate dehydrogenase

► The E1 subunit of pyruvate dehydrogenase (PDH) complex is a major partner of EIIA\Ntr. ► Non-phosphorylated EIIANtr inhibits pyruvate dehydrogenase of P. putida in vivo. ► Manipulating PTSNtr may help engineering bacteria for environmental bioremediation.