کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10801098 | 1054728 | 2005 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A new microperoxidase from Marinobacter hydrocarbonoclasticus
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
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![عکس صفحه اول مقاله: A new microperoxidase from Marinobacter hydrocarbonoclasticus A new microperoxidase from Marinobacter hydrocarbonoclasticus](/preview/png/10801098.png)
چکیده انگلیسی
The preparation and characterization of a new microperoxidase obtained from proteinase K-treated cytochrome c552 from Marinobacter hydrocarbonoclasticus (previously known as Pseudomonas nautica) are presented. This microperoxidase (MMP-5) has novel structural properties relative to previously reported microperoxidases, as the two intervening amino acid (X) residues within the consensual CXXCH c-type heme binding motif are missing, yielding a heme-pentapeptide with increased solubility in aqueous solvents and a 1-2 order of magnitude higher stability of the monomeric state relative to canonical microperoxidases. The electronic spectra in the near-UV and visible regions have been studied as a function of MMP-5 concentration and pH. The spectroscopic properties of MMP-5 are typical of microperoxidases with high-spin hexa- or pentacoordinate heme species dominant in the 1-8 pH range and low-spin states prevailing at higher pH values. In the presence of hydrogen peroxide, MMP-5 displays peroxidatic activities towards several compounds.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - General Subjects - Volume 1725, Issue 1, 30 August 2005, Pages 71-80
Journal: Biochimica et Biophysica Acta (BBA) - General Subjects - Volume 1725, Issue 1, 30 August 2005, Pages 71-80
نویسندگان
Lorenzo Caputi, Alessandra Di Tullio, Luana Di Leandro, Francesco De Angelis, Francesco Malatesta,