کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10801109 | 1054729 | 2005 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Binding of the bioactive component Jatrorrhizine to human serum albumin
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
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چکیده انگلیسی
The interaction between Jatrorrhizine with human serum albumin (HSA) were studied by fluorescence quenching technique, circular dichroism (CD) spectroscopy, and Fourier transform infrared (FT-IR) spectroscopy. Fluorescence data revealed the presence of a single class of binding site on HSA and its binding constants (K) are 7.278Ã104, 6.526Ã104, and 5.965Ã104 L·molâ1 at 296, 303, and 310 K, respectively. The CD spectra and FT-IR spectra have proved that the protein secondary structure changed in the presence of Jatrorrhizine in aqueous solution. The effect of common ions on the binding constants was also investigated. In addition, the thermodynamic functions standard enthalpy (ÎH0) and standard entropy (ÎS0) for the reaction were calculated to be â10.891 kJ·molâ1 and 56.267 J·molâ1 Kâ1, according to the van't Hoff equation. These data indicated that hydrophobic and electrostatic interactions played a major role in the binding of Jatrorrhizine to HSA. Furthermore, the displacement experiments indicated that Jatrorrhizine could bind to the site I of HSA, which was also in agreement with the result of the molecular modeling study.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - General Subjects - Volume 1722, Issue 1, 11 February 2005, Pages 15-21
Journal: Biochimica et Biophysica Acta (BBA) - General Subjects - Volume 1722, Issue 1, 11 February 2005, Pages 15-21
نویسندگان
Ying Li, Wenying He, Jiaqin Liu, Fenling Sheng, Zhide Hu, Xingguo Chen,