کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10801892 | 1055647 | 2015 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Structural effects of Mg2Â + on the regulatory states of three neuronal calcium sensors operating in vertebrate phototransduction
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
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چکیده انگلیسی
Our results confirmed that Mg2Â + is unable to trigger the typical Ca2Â +-induced conformational change of recoverin (myristoyl switch) while it decreases its thermal stability. Interestingly, Mg2Â + seems to affect the conformation of GCAP2 both at high and low [Ca2Â +], however the variations are more substantial for myristoylated GCAP2 in the absence of Ca2Â +. GCAP1 is responsive to Mg2Â + only in its low [Ca2Â +] state and Mg2Â +-GCAP1 tertiary structure slightly differs from both apo and Ca2Â +-bound states. Finally, MD simulations suggest that the GCAP1 state harboring one Mg2Â + ion bound to EF2 acquires structural characteristics that are thought to be relevant for the activation of the guanylate cyclase. Moreover, all the putative Mg2Â +-bound states of myristoylated GCAP1 are structurally less flexible than Ca2Â +-bound states. GCAP1 acquires a more compact tertiary structure that is less accessible to the solvent, thereby inducing a different conformation to the myristoyl moiety, which might be crucial for the activation of the guanylate cyclase. This article is part of a Special Issue entitled: 13th European Symposium on Calcium.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Molecular Cell Research - Volume 1853, Issue 9, September 2015, Pages 2055-2065
Journal: Biochimica et Biophysica Acta (BBA) - Molecular Cell Research - Volume 1853, Issue 9, September 2015, Pages 2055-2065
نویسندگان
Valerio Marino, Stefan Sulmann, Karl-Wilhelm Koch, Daniele Dell'Orco,