کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10802032 | 1055652 | 2015 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Role of actin filaments in fusopod formation and osteoclastogenesis
ترجمه فارسی عنوان
نقش فیبرهای اکتین در تشکیل فوزوپود و استئوکلستوژنز
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کلمات کلیدی
BMMRac1M-CSFsRANKLDC-STAMPTRAcPOsteoclast - استخوانکاه، استئوکلاستtartrate-resistant acid phosphatase - اسید فسفاتاز مقاوم در برابر تارتاتmacrophage colony stimulating factor - فاکتور تحریک کننده کلون ماکروفاژactin filaments - فیبرهای اکتینdendritic cell-specific transmembrane protein - پروتئین پروتئین غشایی خاص سلول دندریتیک
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
چکیده انگلیسی
Cell fusion process is a critical, rate-limiting step in osteoclastogenesis but the mechanisms that regulate fusopod formation are not defined. We characterized fusopod generation in cultured pre-osteoclasts derived from cells stably transfected with a plasmid that expressed a short, actin filament binding peptide (Lifeact) fused to mEGFP that enables localization of actin filaments in living cells. Fusion was initiated at fusopods, which are cell extensions of width > 2 μm and that are immunostained for myosin-X at the extension tips. Fusopods formed at the leading edge of larger migrating cells and from the tail of adjacent smaller cells, both of which migrated in the same direction. Staining for DC-STAMP was circumferential and did not localize to cell-cell fusion sites. Compared with wild-type cells, monocytes null for Rac1 exhibited 6-fold fewer fusopods and formed 4-fold fewer multinucleated osteoclasts. From time-lapse images we found that fusion was temporally related to the formation of coherent and spatially isolated bands of actin filaments that originated in cell bodies and extended into the fusopods. These bands of actin filaments were involved in cell fusion after approaching cells formed initial contacts. We conclude that the formation of fusopods is regulated by Rac1 to initiate intercellular contact during osteoclastogenesis. This step is followed by the tightly regulated assembly of bands of actin filaments in fusopods, which lead to closure of the intercellular gap and finally, cell fusion. These novel, actin-dependent processes are important for fusion processes in osteoclastogenesis.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Molecular Cell Research - Volume 1853, Issue 7, July 2015, Pages 1715-1724
Journal: Biochimica et Biophysica Acta (BBA) - Molecular Cell Research - Volume 1853, Issue 7, July 2015, Pages 1715-1724
نویسندگان
Yongqiang Wang, Patricia Joyce Brooks, Janet Jinyoung Jang, Alexandra Shade Silver, Pamma D. Arora, Christopher A. McCulloch, Michael Glogauer,