کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10802051 | 1055653 | 2015 | 13 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Role of the focal adhesion protein TRIM15 in colon cancer development
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کلمات کلیدی
YFPEGFDMEMFBSDAPIECMGFPGSTVASPTRIM4′,6-diamidino-2-phenylindole - 4 '، 6-دیامیدینو-2-فنیلینولDulbecco's modified Eagle's medium - Medal of Eagle اصلاح شده DulbeccoRing - حلقهColon cancer - سرطان کولونfetal bovine serum - سرم جنین گاوepidermal growth factor - عامل رشد اپیدرمیVasodilator-stimulated phosphoprotein - فسفوپروتئین تحریک شده با واسوادولاتورCell migration - مهاجرت سلولیYellow Fluorescence Protein - پروتئین فلورسانس زردGreen fluorescence protein - پروتئین فلورسانس سبزFocal adhesion - چسبندگی کانونیreally interesting new gene - ژن جدید واقعا جالبCortactin - کورتکتینglutathione S-transferase - گلوتاتیون S-ترانسفراز
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The tripartite motif containing (TRIM) proteins are a large family of proteins that have been implicated in many biological processes including cell differentiation, apoptosis, transcriptional regulation, and signaling pathways. Here, we show that TRIM15 co-localized to focal adhesions through homo-dimerization and significantly suppressed cell migration. Domain mapping analysis indicated that B-box2 and PRY domains were essential for TRIM15 localization to focal adhesions and inhibition of cell migration. Our protein-protein interaction screen of TRIM15 with the integrin adhesome identified several TRIM15 interacting proteins including coronin 1B, cortactin, filamin binding LIM protein1, and vasodilator-stimulated phosphoprotein, which are involved in actin cytoskeleton dynamics. TRIM15 expression was tissue-restricted and downregulated in colon cancer. Level of TRIM15 expression was associated with colon cancer cell migration, as well as both in vitro and in vivo tumor growth. These data provide novel insights into the role of TRIM15 as an additional component of the integrin adhesome, regulating cell migration, and suggest that TRIM15 may function as a tumor suppressor of colon cancer.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Molecular Cell Research - Volume 1853, Issue 2, February 2015, Pages 409-421
Journal: Biochimica et Biophysica Acta (BBA) - Molecular Cell Research - Volume 1853, Issue 2, February 2015, Pages 409-421
نویسندگان
Ok-Hee Lee, Jinkyoung Lee, Keun Ho Lee, Yun Mi Woo, Ju-Hee Kang, Ho-Geun Yoon, Soo-Kyung Bae, Zhou Songyang, Seung Hyun Oh, Youngsok Choi,