In vivo functional calcium imaging of induced or spontaneous activity in the fly brain using a GFP-apoaequorin-based bioluminescent approach

► In-vivo bioluminescence recording of induced or spontaneous activity in the brain ► We have recorded odor-induced Ca2+-activity in the mushroom-bodies. ► Two peaks of spontaneous Ca2+-activity in the mushroom-bodies occur during the night. ► Recording overall (pan-neuronal) spontaneous brain activity during long term period ► Recorded spontaneous activity in glial cells is cell autonomous.