A general framework to characterize inhibitors of calmodulin: Use of calmodulin inhibitors to study the interaction between calmodulin and its calmodulin binding domains

► General strategy finding and characterizing antagonists of protein-protein interactions ► Use of fluorescence polarization to study CaM/target peptide interactions ► CaM antagonists differentiate CaM target peptides complexes. ► New avenue for CaM pharmacology