Phosphorylation and nitration of tyrosine residues affect functional properties of Synaptophysin and Dynamin I, two proteins involved in exo-endocytosis of synaptic vesicles

► Peroxynitrite affects functions of synaptic proteins involved in exo-endocytosis. ► Phosphorylated Tyr273 in C-terminal tail of Synaptophysin binds to and activates c-src. ► Formation of Synaptophysin/Dynamin I complex is impaired by peroxynitrite. ► Peroxynitrite inhibits Dynamin I GTPase activity and self-assembly. ► We identified in Dynamin I one nitration site at Tyr354 using MS/MS analysis.