KLF4 and SOX9 transcription factors antagonize β-catenin and inhibit TCF-activity in cancer cells

► A search for beta-catenin-interacting partners was proposed. ► Sox9 and KLF4 transcription factors were revealed to be potent in inhibiting beta-catenin interaction with TCF probe. ► Sox9 and KLF4 reduced both TCF-binding and activity in many cancer cells lines. ► We are reporting that these transcription factors (Sox9/KLF4) bind to beta-catenin and reduce its interaction with TCF4. ► Sox9 and KLF4 are good candidates to suppress tumor progression.