N-terminal palmitoylation is required for Toxoplasma gondii HSP20 inner membrane complex localization

► TgHSP20 is synthesized and is palmitoylated in three cysteine residues. ► N-terminal palmitoylation determines its IMC localization. ► Incomplete palmitoylation causes TgHSP20 to partially accumulate in a membranous structure. ► TgHSP20 palmitoylation is not responsible for its interaction with daughter cells IMCs.