Identifying protein partners of CLN8, an ER-resident protein involved in neuronal ceroid lipofuscinosis

► CLN8p-protein interactions identified by a split-ubiquitin MYTH library screen ► Putative partners identified: VAPA, c14orf1/hERG28, STX8, GATE16, BNIP3 and BNIP3L. ► Co-immunoprecipitation and co-localization assays support some interactions. ► These partners predict roles of CLN8 in four main biological processes. ► Lipid synthesis/transport, vesicular trafficking, autophagy/mitophagy, apoptosis