Phosphorylation of dynamin II at serine-764 is associated with cytokinesis

► DynII is phosphorylated at the onset of mitosis by Cdk1 at Ser-764. ► Ser-764 phosphorylation does not play a role in clathrin-mediated and bulk endocytosis. ► DynII mitotic centrosome localisation is not dependent on Ser-764 phosphorylation. ► Ser-764 phosphorylation regulates dynII midbody localisation. ► Phospho-dynII-Ser-764 is associated with completion of cytokinesis.