Release of podocalyxin into the extracellular space

► Podocalyxin was detected in the Golgi, plasma membrane and as cytoplasmic vesicles. ► Podocalyxin-containing vesicles seems to move along the filopodia to finally be excreted. ► Podocalyxin was detected in the insoluble and soluble fractions of extracellular medium. ► Insoluble extracellular Podocalyxin was released of as a cargo of microvesicles. ► Soluble extracellular podocalyxin was enhanced by stimulation of metalloproteinases.