کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10803009 | 1055762 | 2008 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Regulation of LPA receptor function by estrogens
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
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چکیده انگلیسی
17β-Estradiol induced LPA1 receptor desensitization in C9 cells stably expressing LPA1 receptors and transiently expressing estrogen receptor α. Such desensitization was evidenced by a reduction in lysophosphatidic acid-mediated Ca2+mobilization and it was associated to receptor phosphorylation and internalization. These effects of 17β-estradiol were rapid (taking place over 5 min) and were blocked by the estrogen receptor antagonist ICI 182780. Similarly, inhibitors of phosphoinositide 3-kinase (wortmannin and LY294002) and of protein kinase C (staurosporine and Gö 6976) blocked 17β-estradiol-induced LPA1 receptor desensitization and phosphorylation. Confocal microscopy evidenced LPA1 receptor internalization in response to 17β-estradiol treatment. Association between LPA1 receptors and protein kinase C α was suggested by co-immunoprecipitation assays. Protein kinase C α was associated with LPA1 receptors in the absence of stimulus and such association further increased in a dynamic fashion in response to 17β-estradiol. The results demonstrated that in C9 cells estrogens modulate LPA1 action through estrogen receptor α with the participation of protein kinase C α and phosphoinositide 3-kinase.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Molecular Cell Research - Volume 1783, Issue 2, February 2008, Pages 253-262
Journal: Biochimica et Biophysica Acta (BBA) - Molecular Cell Research - Volume 1783, Issue 2, February 2008, Pages 253-262
نویسندگان
Aliesha González-Arenas, S. Eréndira Avendaño-Vázquez, Alejandro Cabrera-Wrooman, Diana Tapia-Carrillo, Fernando Larrea, RocÃo GarcÃa-Becerra, J. Adolfo GarcÃa-Sáinz,