کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10803009 1055762 2008 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Regulation of LPA receptor function by estrogens
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Regulation of LPA receptor function by estrogens
چکیده انگلیسی
17β-Estradiol induced LPA1 receptor desensitization in C9 cells stably expressing LPA1 receptors and transiently expressing estrogen receptor α. Such desensitization was evidenced by a reduction in lysophosphatidic acid-mediated Ca2+mobilization and it was associated to receptor phosphorylation and internalization. These effects of 17β-estradiol were rapid (taking place over 5 min) and were blocked by the estrogen receptor antagonist ICI 182780. Similarly, inhibitors of phosphoinositide 3-kinase (wortmannin and LY294002) and of protein kinase C (staurosporine and Gö 6976) blocked 17β-estradiol-induced LPA1 receptor desensitization and phosphorylation. Confocal microscopy evidenced LPA1 receptor internalization in response to 17β-estradiol treatment. Association between LPA1 receptors and protein kinase C α was suggested by co-immunoprecipitation assays. Protein kinase C α was associated with LPA1 receptors in the absence of stimulus and such association further increased in a dynamic fashion in response to 17β-estradiol. The results demonstrated that in C9 cells estrogens modulate LPA1 action through estrogen receptor α with the participation of protein kinase C α and phosphoinositide 3-kinase.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Molecular Cell Research - Volume 1783, Issue 2, February 2008, Pages 253-262
نویسندگان
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