کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10803131 | 1055795 | 2005 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
CCAAT/enhancer binding protein α maintains the ability of insulin-stimulated GLUT4 translocation in 3T3-C2 fibroblastic cells
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
In 3T3-L1 preadipocytes, hormonal induction causes adipose conversion and facilitates the expression of insulin-sensitive glucose transporter, GLUT4. Evidence has accumulated that, in 3T3-L1 preadipocytes, the formation of GLUT4 storage vesicle and its translocation to plasma membrane precede both lipid accumulation and expression of GLUT4 and C/EBPα, a key transcription factor for adipose differentiation. On the other hand, 3T3-C2 fibroblastic cells, a subline of 3T3-L1, follow adipogenic process till mitotic clonal expansion stage (2 days after hormonal induction), but do not proceed to terminal differentiation stage (8 days after the induction), resulting in a lack of adipose conversion and GLUT4 expression. Here we show that, when myc-tagged GLUT4 was retrovirally expressed in 3T3-C2 cells, insulin-stimulated GLUT4 translocation did occur on day 2 after the induction. On day 8 after the induction, however, neither GLUT4 translocation nor the expression of C/EBPα was observed. We also created 3T3-C2 cells stably expressing both myc-tagged GLUT4 and C/EBPα, demonstrating that co-expressed cells showed insulin-stimulated GLUT4 translocation on day 8 after the induction, as well as adipose conversion coupling with PPARγ expression. Our results provide evidence that C/EBPα has the potential to maintain the ability of insulin-stimulated GLUT4 translocation in C/EBPα-deficient 3T3-C2 fibroblastic cells.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Molecular Cell Research - Volume 1745, Issue 1, 15 August 2005, Pages 38-47
Journal: Biochimica et Biophysica Acta (BBA) - Molecular Cell Research - Volume 1745, Issue 1, 15 August 2005, Pages 38-47
نویسندگان
Muneya Fujimoto, Hiroaki Masuzaki, Yuji Yamamoto, Nobuyoshi Norisada, Makoto Imori, Masako Yoshimoto, Tsutomu Tomita, Tomohiro Tanaka, Kayoko Okazawa, Junji Fujikura, Hideki Chusho, Ken Ebihara, Tatsuya Hayashi, Kiminori Hosoda, Gen Inoue, Kazuwa Nakao,