Screening system for orthogonal suppressor tRNAs based on the species-specific toxicity of suppressor tRNAs

▶ Suppressor tRNA proved species-specific toxic to host cells. ▶ Species-specific toxicity of suppressor tRNA is dependent on its orthogonality. ▶ The species-specific toxicity could be used to screen orthogonal suppressor tRNA. ▶ A convenient screening system for orthogonal suppressor tRNA was developed. ▶ It could serve as a general platform for generating tRNA/aaRS pairs.