Structural and functional characterization of a plant S-nitrosoglutathione reductase from Solanum lycopersicum

▶ S-nitrosoglutathione reductase participates in the homeostasis of S-nitrosothiols. ▶ The enzyme catalyses NADH-dependent reduction of S-nitrosoglutathione. ▶ Structures of the apoenzyme and of the enzyme were solved up to 1.9 Ǻ resolution. ▶ Coenzyme binding is associated with the active site zinc movement. ▶ Tomato protein has different compositions of anion-binding pocket compared to human.