Deciphering the molecular structure of cryptolepain in organic solvents

► Cryptolepain is a serine protease isolated from the latex of Cryptolepis buchanani. ► Organic solvents induce molten globule state in cryptolepain under acidic conditions. ► The unfolding of the enzyme in organic solvent reveals the molecular structure. ► Cryptolepain molecule contains two domains which unfold sequentially.