A novel Kazal-type trypsin inhibitor from the skin secretion of the Central American red-eyed leaf frog, Agalychnis callidryas

► First identification of a Kazal-type trypsin inhibitor from frog skin. ► Establishment of high potency and relatedness to other Kazal inhibitors.► Predicted mature peptide located in HPLC fractions of skin secretion and its primary structure confirmed. ► Peptide and encoding precursor cDNA derived from same skin secretion sample.