Biochemical characterization and comparative analysis of two distinct serine proteases from Bothrops pirajai snake venom

► We purified two serine proteases (BpirSP27/SP41) from Bothrops pirajai snake venom. ► Enzymes showed major differences in their sequences, masses and N-glycosylation levels. ► BpirSP27 acted on the Bβ chain and BpirSP41 on both Aα/Bβ chains of fibrinogen. ► BpirSP41 presented higher coagulant and fibrinolytic potential than BpirSP27. ► BpirSP27 induced aggregation of washed platelets to a greater extent than BpirSP41.