In silico and in vitro characterization of phospholipase A2 isoforms from soybean (Glycine max)

► Five sequences of secretory sPLA2 from soybean (Glycine max) are reported. ► The Ca2+ binding loop and the active site motif are similar to others plants enzymes. ► The enzymes belong to XIA and XIB subgroups according to PLA2 superfamily. ► Recombinant folded GmsPLA2-XIA-1 has activity against substrate in mixed micelles. ► Molecular dynamics indicate that Ca2+ plays an important role in PLA2 stability.