Functional peptidomics of amphibian skin secretion: A novel Kunitz-type chymotrypsin inhibitor from the African hyperoliid frog, Kassina senegalensis

► Identification and structural characterisation of the first chymotrypsin inhibitor from amphibian skin secretion. ► Establishment of its identity as a Kunitz-type inhibitor/toxin. ► Use of a functional peptidomics approach involving peptide sequencing, molecular cloning and biological evaluation. ► Use of Orbitrap high-resolution mass spectrometry for accurate mass determination. ► Peptide and encoding precursor cDNA derived from same skin secretion sample.