Ras and Rap1 govern spatiotemporal dynamic of activated ERK in pituitary living cells

► EGF and VIP specifically control spatiotemporal ERK dynamic. ► Ras is specifically required for EGF-activated ERK nuclear pool. ► Ras is required for both cytoplasmic and nuclear pools of VIP-activated ERK. ► Rap1 is exclusively involved in VIP-activated ERK nuclear pool. ► Ras and Rap1 are differentially involved in ERK nuclear translocation.