کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10815487 | 1058476 | 2014 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
CaV1.3 L-type channels, maxiK Ca2Â +-dependent K+ channels and bestrophin-1 regulate rhythmic photoreceptor outer segment phagocytosis by retinal pigment epithelial cells
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کلمات کلیدی
EOGRPESOCEDHPintracellular Ca2 + concentrationPhotoreceptor outer segmentLight peakMer tyrosine kinase[Ca2 +]i - [Ca2 +] ielectro-oculogram - الکتریک oculogramretinal pigment epithelium - اپیتلیوم رنگدانه شبکیهDihydropyridines - دی هیدروپیریدینMERTK - زیراPhagocytosis - فاگوسیتوز یا بیگانهخواری pos - پست
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Phagocytosis of shed photoreceptor outer segments by the retinal pigment epithelium (RPE) is critical for maintenance of visual function. Because changes in intracellular Ca2Â + regulate phagocytosis, we studied in vitro the impact of different ion channels in addition to mice deficient for Cav1.3 L-type Ca2+ channels (Ca1.3â/â) and maxiK Ca2+-dependent K+ channels (BKâ/â). The knockdown of Bestrophin-1 protein, a regulator of intracellular Ca2+ homeostasis, affected phagocytosis in porcine RPE cultures. Blockage of voltage-gated L-type channels by (+)BayK8644 inhibitor reduced phagocytosis in vitro, in contrast L-type activation by (â)BayK8644 had no impact. The expression rate of Cav1.3, the predominant L-type Ca2Â + channel in RPE cells, varied at different times of day. CaV1.3â/â RPE lacked peak phagocytic activity following morning photoreceptor shedding in wild-type RPE and retained a higher number of phagosomes at a later time of day. The BK-channel blocker paxilline lowered phagocytosis in RPE cultures in a concentration-dependent manner. BKâ/â RPE in vivo retained phagocytic capability but this activity, which is normally well synchronized with circadian photoreceptor shedding, shifted out of phase. Retinae of older BKâ/â mice showed shortened photoreceptor outer segments and diminished rhodopsin content. Store-operated Ca2Â + channels Orai-1 did not affect phagocytosis in cultured RPE. TRPV channel inhibition by ruthenium-red reduced phagocytosis, whereas activation at high concentrations of 2-APB increased phagocytosis. Our data demonstrate essential roles for bestrophin-1, BK, TRPV and L-type channels in regulating retinal phagocytosis. These data indicate further the importance of BK and CaV1.3 for rhythmic phagocytic activity synchronized with photoreceptor shedding.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cellular Signalling - Volume 26, Issue 5, May 2014, Pages 968-978
Journal: Cellular Signalling - Volume 26, Issue 5, May 2014, Pages 968-978
نویسندگان
Claudia Müller, Néstor Más Gómez, Peter Ruth, Olaf StrauÃ,