Bone morphogenetic protein (BMP) signaling regulates mitotic checkpoint protein levels in human breast cancer cells

► BMP signaling inhibition by Dorsomorphin (DM) abrogates mitotic arrest. ► TGF-β signaling inhibition by SB431542 does not abrogate mitotic arrest. ► DM, noggin and SMAD1, 5, 4 knockdown downregulate checkpoint proteins. ► BMP and Activin signaling regulate mitotic checkpoint protein levels differentially. ► Overexpressing some mitotic genes significantly rescues mitotic defect caused by DM.