Identification of Ser/Thr phosphorylation sites in the C2-domain of phospholipase C γ2 (PLCγ2) using TRPM7-kinase

► PLCγ2 interacts with the Mg2 +-sensing channel-kinase TRPM7. ► The C2-domain of PLCγ2 is phosphorylated by TRPM7's Ser/Thr kinase. ► TRPM7-mediated PLCγ2 phosphorylation reveals two novel phospho-sites, T1045 and S1164. ► PLCγ2-S1164A mutation results in diminished BCR-mediated Ca2 +-signal under low Mg2 +.