Small GTPase Rho regulates R-cadherin through Dia1/profilin-1

► We identified previously unappreciated connection between Rho and R-cadherin. ► Rho regulates R-cadherin adherens junction via Dia1 and profilin-1. ► Data suggested an inverse relationship between EGFR signaling and R-cadherin. ► Data indicated R-cadherin as a critical regulator of epithelial phenotype.