Preanalytical, analytical (DiaSorin LIAISON) and clinical variables potentially affecting the 25-OH Vitamin D estimation

► The importance of vitamin D (25-OHD) estimation renders method validation mandatory. ► Results confirm LIAISON 25-OHD assay as a useful tool for 25-OHD estimation. ► Hypovitaminosis D is common among Italian adults. ► 25-OHD appears associated with different cardiovascular risk factors. ► Season of examination may recall bias in the assessment of 25-OHD.