Measurement of intracellular vitamin C levels in human lymphocytes by reverse phase high performance liquid chromatography (HPLC)

The proposed HPLC method offers a reliable and reproducible technique for the quantification of intracellular vitamin C. Lymphocyte samples can be rapidly prepared and represent a more homogeneous tissue sample source for intracellular vitamin C measurement as compared to serum. To ensure stability, lymphocyte lysates should be prepared and stored at or below −20°C within 2 h of blood collection.