کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10818850 | 1060270 | 2013 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Rho participates in chemoreceptor-induced changes in morphology to hair bundle mechanoreceptors of the sea anemone, Nematostella vectensis
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
PBSCaM-kinaseCyclic-AMPGDINematostella vectensispKaGEFTBSTIP3GPCRBSA - BSAG-protein coupled receptors - G-پروتئین گیرنده های متصل شدهInositol 1,4,5-triphosphate - Inositol 1،4،5-تری فسفاتTris-buffered saline and Tween 20 - Tris-buffered saline و Tween 20bovine serum albumin - آلبومین سرم گاوgap junctions - اتصالات شکاف دارN-acetylneuraminic acid - اسید N-استیلون اورامینیکGDP dissociation inhibitor - بازدارنده جداسازی تولید ناخالص داخلیRho - روguanine nucleotide exchange factor - فاکتور تبادل نوکلئوتید گوانینPhosphate buffered saline - فسفات بافر شورNanA - ناناCalmodulin-dependent kinase - وابسته به کالمدولین وابسته به کینازCalcineurin - کلسینورین
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Adjustable hair bundle mechanoreceptors located on anemone tentacles detect movements of nearby, swimming prey. The hair bundles are formed by numerous actin-based stereocilia that converge onto a single, central kinocilium. Interestingly, morphological and functional changes to the hair bundles are induced by activating chemoreceptors that bind prey-derived N-acetylated sugars and proline, respectively. Morphological changes to the hair bundles involve alterations to the actin cytoskeleton of stereocilia. A pharmacological activation of Rho induces hair bundles to elongate to lengths comparable to those normally induced by exposure to N-acetylneuraminic acid (NANA) and prevents shortening of hair bundles normally induced by proline. Rho inhibition prevents NANA-induced elongation, but does not prevent proline-induced shortening of hair bundles. Western blots feature a band similar in mass to that predicted for a Rho homolog in the genome of Nematostella. Immunocytochemistry localizes Rho in stereocilia of the hair bundle. Anemone hair bundles arise from multicellular complexes. Data from experiments using heptanol, a gap junction uncoupler, indicate that cell-cell communication is required in order for activated chemoreceptors to induce morphological changes to the hair bundles.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology - Volume 165, Issue 2, June 2013, Pages 139-148
Journal: Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology - Volume 165, Issue 2, June 2013, Pages 139-148
نویسندگان
Kathryn M. Allaire, Glen M. Watson,