Go hybrid: EM, crystallography, and beyond

► Multiple structural methodologies must be combined to fully describe the macromolecular rearrangements that underlie function. ► Interpretation of cryo-EM studies is greatly aided by labeling and biochemical data when atomic structures are lacking. ► Time-resolved methods like SAXS can identify structural transitions in a conformational pathway, for more detailed study. ► Elucidation of dynamic pathways is aided by molecular dynamics while constraining movements to the cryoEM envelope.