NMR methods for structural studies of large monomeric and multimeric proteins

- Large monomeric proteins suffer from spectral crowding.
- Nonuniform sampling of 3D and 4D data optimizes spectral resolution.
- Selective methyl labeling and tailored NMR experiments provide distance constraints.
- Large multimeric proteins are studied with divide and conquer approach.
- Multimeric proteins are studied by combining NMR methods with other techniques.