کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10822751 1061703 2005 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Using nanoliter plugs in microfluidics to facilitate and understand protein crystallization
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Using nanoliter plugs in microfluidics to facilitate and understand protein crystallization
چکیده انگلیسی
Protein crystallization is important for determining protein structures by X-ray diffraction. Nanoliter-sized plugs - aqueous droplets surrounded by a fluorinated carrier fluid - have been applied to the screening of protein crystallization conditions. Preformed arrays of plugs in capillary cartridges enable sparse matrix screening. Crystals grown in plugs inside a microcapillary may be analyzed by in situ X-ray diffraction. Screening using plugs, which are easily formed in PDMS microfluidic channels, is simple and economical, and minimizes consumption of the protein. This approach also has the potential to improve our understanding of the fundamentals of protein crystallization, such as the effect of mixing on the nucleation of crystals.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Current Opinion in Structural Biology - Volume 15, Issue 5, October 2005, Pages 548-555
نویسندگان
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