کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10822794 | 1061708 | 2005 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Virus maturation: dynamics and mechanism of a stabilizing structural transition that leads to infectivity
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موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
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چکیده انگلیسی
For many viruses, the final stage of assembly involves structural transitions that convert an innocuous precursor particle into an infectious agent. This process - maturation - is controlled by proteases that trigger large-scale conformational changes. In this context, protease inhibitor antiviral drugs act by blocking maturation. Recent work has succeeded in determining the folds of representative examples of the five major proteins - major capsid protein, scaffolding protein, portal, protease and accessory protein - that are typically involved in capsid assembly. These data provide a framework for detailed mechanistic investigations and elucidation of mutations that affect assembly in various ways. The nature of the conformational change has been elucidated: it entails rigid-body rotations and translations of the arrayed subunits that transfer the interactions between them to different molecular surfaces, accompanied by refolding and redeployment of local motifs. Moreover, it has been possible to visualize maturation at the submolecular level in movies based on time-resolved cryo-electron microscopy.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Current Opinion in Structural Biology - Volume 15, Issue 2, April 2005, Pages 227-236
Journal: Current Opinion in Structural Biology - Volume 15, Issue 2, April 2005, Pages 227-236
نویسندگان
Alasdair C Steven, J Bernard Heymann, Naiqian Cheng, Benes L Trus, James F Conway,