A modified host-cell reactivation assay to quantify DNA repair capacity in cryopreserved peripheral lymphocytes

► Quantitation of DNA repair levels in cryopreserved PBLs. ► The method combines: nucleoporation, a GFP-based reporter gene and flow cytometry. ► Accurate discrimination among different Xeroderma pigmentosum phenotypes. ► High intra- and inter-experimental DNA repair levels reproducibility. ► Reliable method for both in vitro and population-based studies.