کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10823594 1061910 2005 12 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Roles of replicative and specialized DNA polymerases in frameshift mutagenesis: Mutability of Salmonella typhimurium strains lacking one or all of SOS-inducible DNA polymerases to 26 chemicals
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Roles of replicative and specialized DNA polymerases in frameshift mutagenesis: Mutability of Salmonella typhimurium strains lacking one or all of SOS-inducible DNA polymerases to 26 chemicals
چکیده انگلیسی
Progression of DNA replication is occasionally blocked by endogenous and exogenous DNA damage. To circumvent the stalling of DNA replication, cells possess a variety of specialized DNA polymerases that replicate through DNA damage. Salmonella typhimurium strain TA1538 has six DNA polymerases and four of them are encoded by damage-inducible SOS genes, i.e. polBST (pol II), dinBST (pol IV), umuDCST (pol V) and samAB. The strain has been used for the detection of a variety of chemical mutagens because of the high sensitivity to −2 frameshift occurring in CGCGCGCG sequence. To assign the role of each DNA polymerase in the frameshift mutagenesis, we have constructed the derivatives lacking one or all of SOS-inducible DNA polymerases and examined the mutability to 26 chemical mutagens. Interestingly, the chemicals could be categorized into four classes: class I whose mutagenicity was reduced by the deletion of dinBST (1-aminoanthracene and other four chemicals); class II whose mutagenicity was reduced by the deletion of either dinBST or umuDCST plus samAB (7,12-dimethylbenz[a]anthracene and other three chemicals); class III whose mutagenicity largely depended on the presence of umuDCST plus samAB (1-N-6-azabenzo[a]pyrene and other three chemicals) and class IV whose mutagenicity was not reduced by deletion of any of the genes encoding SOS-inducible DNA polymerases (Glu-P-1 and other 12 chemicals). Deletion of polBST reduced by 30-60% the mutagenicity of six chemicals of classes II and III. These results suggest that multiple DNA polymerases including the replicative DNA polymerase, i.e. DNA polymerase III holoenzyme, play important roles in chemically induced −2 frameshift and also that different sets of DNA polymerases are engaged in the translesion bypass of different DNA lesions.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: DNA Repair - Volume 4, Issue 10, 28 September 2005, Pages 1160-1171
نویسندگان
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