Binding of the UvrB dimer to non-damaged and damaged DNA: Residues Y92 and Y93 influence the stability of both subunits

In addition to preventing binding to non-damaged DNA, the Y92 and Y93 residues appear also important for making specific contacts (of B1) with the damaged site. We show that the concerted action of the two tyrosines lead to a conformational change in the DNA surrounding the lesion, which is required for the 3′ incision reaction.