کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10824302 | 1062352 | 2005 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Prophenoloxidase binds to the surface of hemocytes and is involved in hemocyte melanization in Manduca sexta
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کلمات کلیدی
PBSFITCTBSManduca sextaImmulectin-2Innate immunity - ایمنی ذاتیTris-buffered saline - تریس بافر شورfluorescein isothiocyanate - فلوئورسین ایسوتیوسیاناتphenoloxidase - فنلوکسیدازPhosphate-buffered saline - محلول نمک فسفات با خاصیت بافریMelanization - ملانازیproPO - نزدیکHemocyte - هموسیتprophenoloxidase - پروپنولوکسیدازEncapsulation - کپسوله سازی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش حشره شناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
In insects, melanotic encapsulation is an important innate immune response against large pathogens or parasites, and phenoloxidase (PO) is a key enzyme in this process. Activation of prophenoloxidase (proPO) to PO is mediated by a serine proteinase cascade. PO has a tendency to adhere to foreign surfaces including hemocyte surfaces. In this study, we showed that in the naïve larvae of the tobacco hornworm Manduca sexta, hemolymph proPO bound to the surface of granulocytes and spherule cells but not to oenocytoids, and about 10% hemocytes had proPO on their surfaces. When larvae were injected with water (injury) or microsphere beads (immune-challenge), hemolymph proPO was activated, and the number of hemocytes with surface proPO/PO increased at 12Â h post-injection, but dropped to the normal level at 24Â h. Hemocyte surface proPO can be activated in vitro, leading to melanization of these hemocytes. The number of melanized hemocytes from the larvae injected with water or microsphere beads significantly increased. We also showed that neither hemocytes nor cell-free plasma alone triggered melanization of immulectin-2-coated agarose beads in vitro. However, agarose beads were effectively melanized by isolated hemocytes in the presence of cell-free plasma. Our results suggest that activation of hemocyte surface proPO may initiate melanization, leading to the systemic melanization of hemocyte capsules.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Insect Biochemistry and Molecular Biology - Volume 35, Issue 12, December 2005, Pages 1356-1366
Journal: Insect Biochemistry and Molecular Biology - Volume 35, Issue 12, December 2005, Pages 1356-1366
نویسندگان
Erjun Ling, Xiao-Qiang Yu,