کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10824376 | 1062361 | 2005 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Molecular cloning and analysis of a novel teratocyte-specific carboxylesterase from the parasitic wasp, Dinocampus coccinellae
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش حشره شناسی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Molecular cloning and analysis of a novel teratocyte-specific carboxylesterase from the parasitic wasp, Dinocampus coccinellae Molecular cloning and analysis of a novel teratocyte-specific carboxylesterase from the parasitic wasp, Dinocampus coccinellae](/preview/png/10824376.png)
چکیده انگلیسی
Teratocytes derived from the embryonic membrane (serosa) of parasitoids are released into the host hemocoel when the parasitoid eggs hatch, where they perform several functions during the post-embryonic stage. A full-length cDNA encoding a putative carboxylesterase was isolated from the teratocytes of Dinocampus coccinellae and was designated as teratocyte-specific carboxylesterase (TSC). It contained an open reading frame of 2571Â bp coding for a protein of 857 amino acids with a calculated molecular mass of 89Â kDa. The deduced amino acid sequence had many structural features that are highly conserved among serine hydrolases including Ser, Glu and His as a catalytic triad, carboxylesterase type-B (FGGNPNSVTLLGYSAG)/ lipase-serine (VTLLGYSAGA) active sites, and six N-glycosylation sites. Interestingly, the mRNA encoding the TSC gene was expressed exclusively in teratocytes but not in the parasitoid larva or in the non-parasitized host. Most notably, the TSC protein was distinguished by an insertion of 294 amino acids towards the N-terminal region and was flanked by carboxylesterase domains. Furthermore, sequence alignment and homology search revealed these additional amino acids to be unique to TSC and the insertion contributed significantly to its molecular mass resulting in a larger protein than other esterases. In addition to sequence analysis, the possible role of TSC in relation to the host (Coccinella septempunctata) and parasitoid (D. coccinellae) system is discussed.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Insect Biochemistry and Molecular Biology - Volume 35, Issue 10, October 2005, Pages 1171-1180
Journal: Insect Biochemistry and Molecular Biology - Volume 35, Issue 10, October 2005, Pages 1171-1180
نویسندگان
Ravikumar Gopalapillai, Keiko Kadono-Okuda, Takashi Okuda,