کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10824935 1063312 2005 21 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Effect of system variables involved in packed column SFC of nevirapine as model analyte using response surface methodology: Application to retention thermodynamics, solute transfer kinetic study and binary diffusion coefficient determination
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Effect of system variables involved in packed column SFC of nevirapine as model analyte using response surface methodology: Application to retention thermodynamics, solute transfer kinetic study and binary diffusion coefficient determination
چکیده انگلیسی
A multifactor optimization technique is successfully applied to study the effect of simultaneously varying the system variables on feasibility of nevirapine analysis by packed column supercritical fluid chromatography (PC-SFC). The optimal conditions were determined with the aid of the response surface methodology using 33 factorial designs. The method is based on methanol-modified carbon dioxide as the mobile phase at flow rate of 3.0 ml/min with elution through a JASCO Finepak SIL-5, [C18 (5-micron, 25 cm × 4.6 mm, i.d.)] column using photodiode array detection. The method has been successfully used to analyze commercial solid dosage form to assess the chromatographic performance of SFC system. The present work briefs the thermodynamic applications of PC-SFC with an emphasis on the results of nevirapine. The foremost of such applications is the determination of solute diffusion coefficient in supercritical mobile phase by Taylor-Aris peak broadening technique.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Biochemical and Biophysical Methods - Volume 64, Issue 2, 31 August 2005, Pages 121-141
نویسندگان
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