کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10825130 1063345 2005 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Synthesis and characterization of a novel reagent containing dansyl group, which specifically alkylates sulfhydryl group: An example of application for protein chemistry
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Synthesis and characterization of a novel reagent containing dansyl group, which specifically alkylates sulfhydryl group: An example of application for protein chemistry
چکیده انگلیسی
To show the usefulness of IADC, the cysteines in glyceraldehyde-3-phosphate dehydrogenase (GAPDH) from chicken muscle were modified with this reagent, and GAPDH was then digested by lysyl endopeptidase. The peptides generated from digestion of IADC-incorporated GAPDH were applied to an anti-dansyl immunoaffinity column. The peptide fragments bound and eluted from the column were separated by HPLC, and the amino acid sequence of each peptide was analyzed, and peptide was identified as the one containing a Cys residue(s). These data showed that IADC is a useful reagent to specifically identify the positions of a Cys residue(s) in proteins.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Biochemical and Biophysical Methods - Volume 63, Issue 1, 29 April 2005, Pages 33-42
نویسندگان
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