کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10826092 | 1064710 | 2011 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Investigation of protein induction in tumour vascular targeted strategies by MALDI MSI
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کلمات کلیدی
PMFVDAAngiogenesis - آنژیوژنزPeptide mass fingerprint - اثر انگشت پپتیدهmass spectrometry imaging - تصویربرداری طیف سنج جرمیHaemorrhage - خونریزیVascular disrupting agent - عامل اختلال عروقیVascular endothelial growth factor - فاکتور رشد اندوتلیال عروقیVascular Endothelial Growth Factor (VEGF) - فاکتور رشد اندوتلیال عروقی (VEGF)Matrix-assisted laser desorption/ionisation - لیزر جذب / یونیزاسیون با کمک ماتریکسMALDI MSI - مالدی MSI
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Investigation of protein induction in tumour vascular targeted strategies by MALDI MSI Investigation of protein induction in tumour vascular targeted strategies by MALDI MSI](/preview/png/10826092.png)
چکیده انگلیسی
Characterising the protein signatures in tumours following vascular-targeted therapy will help determine both treatment response and resistance mechanisms. Here, mass spectrometry imaging and MS/MS with and without ion mobility separation have been used for this purpose in a mouse fibrosarcoma model following treatment with the tubulin-binding tumour vascular disrupting agent, combretastatin A-4-phosphate (CA-4-P). Characterisation of peptides after in situ tissue tryptic digestion was carried out using Matrix-Assisted Laser Desorption/Ionisation-Mass Spectrometry (MALDI-MS) and Matrix-Assisted Laser Desorption/Ionisation-Ion Mobility Separation-Mass Spectrometry Imaging (MALDI IMS-MSI) to observe the spatial distribution of peptides. Matrix-Assisted Laser Desorption/Ionisation-Ion Mobility Separation-Tandem Mass Spectrometry (MALDI-IMS-MS/MS) of peaks was performed to elucidate any pharmacological responses and potential biomarkers. By taking tumour samples at a number of time points after treatment gross changes in the tissue were indicated by changes in the signal levels of certain peptides. These were identified as arising from haemoglobin and indicated the disruption of the tumour vasculature. It was hoped that the use of PCA-DA would reveal more subtle changes taking place in the tumour samples however these are masked by the dominance of the changes in the haemoglobin signals.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Methods - Volume 54, Issue 4, August 2011, Pages 442-453
Journal: Methods - Volume 54, Issue 4, August 2011, Pages 442-453
نویسندگان
L.M. Cole, M-C. Djidja, J. Bluff, E. Claude, V.A. Carolan, M. Paley, G.M. Tozer, M.R. Clench,