کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10826169 1064728 2010 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Examining the contents of isolated Xenopus germinal vesicles
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Examining the contents of isolated Xenopus germinal vesicles
چکیده انگلیسی
One can manually isolate the giant oocyte nucleus or germinal vesicle (GV) of Xenopus from a living oocyte with nothing more complicated than jewelers' forceps and a dissecting microscope. Similarly, one can remove the nuclear envelope by hand and allow the lampbrush chromosomes and other nuclear organelles to spread on a microscope slide. After centrifugation, the nuclear contents adhere tightly to the slide, where they can be subjected to immunostaining or fluorescent in situ hybridization for visualization by conventional or confocal microscopy. Preparations of isolated GV contents reveal details of nuclear structure that are almost impossible to attain by more conventional techniques.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Methods - Volume 51, Issue 1, May 2010, Pages 45-51
نویسندگان
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