High throughput construction and small scale expression screening of multi-tag vectors in Escherichia coli

► A suite of N-terminal hexa-histidine tagged fusion protein vectors is presented. ► 9 fusion proteins can be made from one PCR product using Infusion™ cloning. ► The effect of fusions on the solubility of 20 proteins in E coli was tested in 2 media. ► The SUMO tag was the most effective at solubilising targets. ► Auto-induction media gave more high level expressers than IPTG induction.