In vitro testing of anthelmintic efficacy of Flemingia vestita (Fabaceae) on carbohydrate metabolism in Rallietina echinobothrida

The root tuber peel of Flemingia vestita has been in use in local traditional medicine against intestinal worm infections in Meghalaya (North-East India). In order to evaluate and authenticate the anthelminitc efficacy of the isoflavones of F. vestita, the root peel extract of this putative plant was tested against several helminth parasites, extensively on Rallietina echinobothrida, with respect to different parameters of these parasites. In this paper, we describe various methods to evaluate the anthelmintic efficacy of this medicinal plant with respect to carbohydrate metabolism in R. echinobothrida at paralytic time caused by the isoflavones of F. vestita. To meet the high energy demand by the parasite due to the anthelmintic stress, glucose breakdown follows the PEPCK-malate pathway in the parasite.