کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10826727 | 1064825 | 2005 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Use of fluorescent sphingolipid analogs to study lipid transport along the endocytic pathway
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
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چکیده انگلیسی
Sphingolipids (SLs) are concentrated at the plasma membrane where they play important roles in cell-cell communication, host-pathogen interactions, and cell signaling events. Our laboratory has used fluorescent SL analogs and SL-binding toxins to elucidate mechanisms by which SLs are internalized by endocytosis and subsequently sorted and transported to various intracellular compartments. These studies have relied on the use of temperature shift protocols, co-localization studies with compartment-specific markers, selective biochemical treatments that inhibit specific endocytic mechanisms, and the expression of dominant negative proteins (e.g., rabs) that block specific steps in transport. These methods are presented here so that they can be utilized by others for the study of endocytic trafficking of lipids and other molecules.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Methods - Volume 36, Issue 2, June 2005, Pages 186-195
Journal: Methods - Volume 36, Issue 2, June 2005, Pages 186-195
نویسندگان
David L. Marks, Raman Deep Singh, Amit Choudhury, Christine L. Wheatley, Richard E. Pagano,